Ayame is introduced as a volunteer for the Black Knights and is usually seen alongside Minase Mutsuki and Ichijiku Hinata.
Ayame watching over Tamaki.
She was present during Viceroy Nunnally's Special Zone where one million Zeroes were exiled from Japan. After being exiled, she appears inside the Ikaruga. She is present during the battles that ensue in the Chinese Federation.
During the final battle, she is seen comforting Tamaki in a hospital after he is defeated by Suzaku in his Lancelot Albion. She respectively refers to him as Senpai.